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Weitwinkelmikroskop

10. AMBIO Nikon Widefield Ti2

Betreiber: Charité

Nutzbar durch: HU | TU | FU | Charité

Nikon Widefield Ti2

Charité Campus Mitte, CCO, Rm. 01-307, Virchowweg 6, 10117 Berlin, Germany

 

MAIN FEATURE: Fast high-throughput multiplex 3D live-cell screening

Configuration

  • Multi-color, long-term time series, multi-position / multi-well plates, large image scan
  • Fully motorized high precision stage for accurate XYZ-repositioning
  • Focus drift correction (Nikon Perfect Focus System)
  • Live-cell incubator (temperature, CO2, humidity, carbogen)
  • Automatic water immersion dispenser (WID) for water immersion objectives for high-resolution on water-based samples
  • Perfusion

Imaging Modes

  • Widefield epifluorescence (DAPI, CFP, GFP, YFP, mCherry, Cy5)
  • Bright-field (transmitted light) for histological slides (color stains)
  • Automated phase contrast for 10x & 20x objectives
  • FRET
  • Interference reflection microscopy (IRM)
  • Polarized light microscopy

Special

  • Fast LED switching/ tuning (triggered acquisition)
  • Fast triggered emission filter wheel for clean multi-color imaging of DAPI, CFP, GFP, YFP, mCherry, and Cy5
  • NIS-Elements AR (Advanced Research) software including modules for:
    • Automated acquisition & feedback microscopy (NIS-Elements JOBS module)
    • 2D & 3D Deconvolution
    • NIS.ai (Artificial Intelligence): denoise, clarify, enhance, segment, convert

LED Illumination (Lumencore, SpectraX)

  • 395/25 nm
  • 438/29 nm
  • 475/28 nm
  • 511/16 nm
  • 555/28 nm
  • 635/22 nm

Detectors

  • For fluorescent samples: sCMOS pco.edge 4.2 LT HQ camera (82% QE, 2048×2048 pixels, 6.5 µm pixel size, 53 fps full frame, up to 500 fps in small area)
  • For stained samples (histology): Nikon DS-Ri2 color CMOS camera (4908×3264 pixels, 6 fps full frame, 45 fps in 1636×1088 pixels)

Emission Filters (λ/Bandwidth (example fluorophore))

  • QUAD
    • 435/33 (DAPI)
    • 520/26 (GFP)
    • 595/38 (mCherry)
    • 695/63 (Cy5)
  • 482/35 (CFP)
  • 540/30 (YFP)
  • 519/26 (GFP)
  • 595/31 (mCherry)
  • 697/60 (Cy5)

Objectives

  • 4x Plan Apo λ Air                             NA 0.2 WD 20,000 µm
  • 10x Plan Fluo Ph1 DL Air                 NA 0.3 WD 15,200 µm
  • 20x Plan Apo Ph2 DM Air                 NA 0.75 WD 1,000 µm
  • 40x Plan Apo λ DIC N2 Air               NA 0.95 WD 250 – 170 µm
  • 40x Apo LWD λS DIC N2 Water        NA 1.15 WD 590 – 610 µm
  • 60x Plan Apo λ DIC N2 Oil               NA 1.4 WD 130 µm

Weiterführende Informationen:

https://bua.openiris.io/Landing/Resource?id=5802
Alle ausklappen

Mögliche Nutzer:innengruppen: Keine Einschränkungen

Nutzungsvoraussetzungen:

The User and PI hereby declare to have understood and accepted the AMBIO User Guidelines and Rules
including the Safety Rules, as available online at: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2025-01-30.pdf. It is further
understood that these Guidelines and Rules may be updated or changed in agreement with the AMBIO
steering committee. Registered Users and PIs will be notified of essential changes (service fees).
Furthermore, the new User and PI have understood that a fee will be charged for the usage of AMBIO services
as outlined in the AMBIO User Guidelines and Rules, and that they are willing and able to pay the fees.

 

Hinweis

New users – access upon training request here.

E-Mail: ambio@charite.de

Anbieter:

Advanced Medical BIOimaging Core Facility

Standort Anbieter:

Campus Charité Mitte (CCM)
CharitéCrossOver (CCO)
Rm 01-306
Virchowweg 6
10117 Berlin
Germany

Standort:

Charité Campus Mitte
CCO Rm. 01-307
Virchowweg 6
10117 Berlin
Germany

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