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Shared Service

Large-scale research equipment and laboratories / Imaging Systems

Microscope

Leica Stellaris 8

Carrier: Charité

Usable by: HU | TU | FU | Charité

Imaging modes

  • Scanning confocal (including XZ direction scans)
  • Wide field (DAPI, FITC, YFP, RHOD, TXR)
  • Video-imaging up to 60 fps
  • TauSense, TauSeparation, TauGating: functional imaging based on arrival times
  • FRAP 
  • Live-cell incubator (temperature, CO2, humidity)
  • Spectral scans
  • Lightning mode (near super-resolution)

Lasers and max. intensities out of 60x oil objective

  • 405 nm 
  • White light laser: 440-700 nm (pulsed)

Emission Filters

Filter λ/Bandwidth
DAPI 460/50
FITC 527/30
YFP 535/30
RHOD 585/40
TXR 630/75

Scanner

  • AOBS scanner

Detectors

  • 2x Power Hybrid Detektor HyD S
  • 2x Power Hybrid Detektor HyD X
  • Transmission light detector (DIC, phase)
  • Spectral detection

Objectives

Mag. Type Immer. NA WD [μm] Info
10x HC PL FLUOTAR Air 0.3 11,000  
20x HC PL FLUOTAR Air 0.55  
20x HC PL APO Water (multi imm) 0.75 670
40x HC PL APO Oil 1.30 240
63x HC PL APO Water 1.20 300 motCorr
2.5x HC PL FLUOTAR Air 0.07 9,200

Special

  • Super Z-galvo stage
  • High precision LMT scanning stage
  • Automatic water immersion for long-term imaging
  • Resonant scanner for fast imaging

Further information:

https://bua.openiris.io/Landing/Resource?id=16807

Possible user: No restrictions

Note

To get access to the microscope please click on the request access icon.

Email: Dr. Anita Balázs (anita.balazs@charite.de)

Provider:

Shared Equipment at Charité

Provider Location:

Berlin

Location:

Charité Campus Virchow Klinikum
AG Mall
Forschungshaus Rm. 1.0516
Forum 4
Augustenburger Platz 1
13353 Berlin
Germany

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