MAIN FEATURE: Confocal laser scanning microscope with galvo and resonant scanners, 4 fluorescence detectors and a 32 PMT-detector array for spectral detection and spectral unmixing. Additionally, time-resolved imaging (FLIM/FCS by PicoQuant) is possible.
- Multi-color, long-term time series, multi-position / multi-well plates, large image scan
- Fully motorized high precision stage for accurate XYZ-repositioning
- Piezo-Z-stage for super-fast and precise focusing & stacking (100 µm range, Mad City Labs)
- Focus drift correction (Nikon Perfect Focus System)
- Live-cell incubator (temperature, CO2, humidity, carbogen)
- Perfusion
Imaging Modes
- Confocal laser scanning (DAPI, GFP, mCherry, Cy5, transmitted light)
- Widefield epifluorescence (DAPI, GFP, mCherry, Cy5)
- Bright-field (transmitted light)
- DIC for 20x air, 40x water & 40x/60x oil objectives
- Spectral scans/ spectral unmixing
- Fluorescence recovery after photobleaching (FRAP), photo activation (PA) & photo conversion (optogenetics)
- FLIM, FLIM-FRET, FCS, FCCS
Special
- Fast switch between galvo and resonant scanners
- NIS-Elements C (Confocal) software including modules for:
- Automated acquisition & feedback microscopy (NIS-Elements JOBS module)
Excitation Lasers
Confocal
- 405 nm
- 488 nm
- 561 nm
- 647 nm
Picosecond-pulsed for FLIM
- 440 nm
- 473 nm
Scanner
- Point scanner (galvo)
- Resonant scanner (30 fps in 1024×1024 pixels, up to 420 fps in 512×32 pixels)
- Pinhole: hexagonal, tunable
Detectors
- Confocal-5-channel
- 2x Hybrid GaAsP PMT (high sensitivity, GFP, mCherry)
- 2x PMT (DAPI, Cy5)
- Transmission light detector (DIC, phase)
- 2x PMA Hybrid detectors in confocal (descanned) detection mode for FLIM/FCS
- Spectral detector (optical diffraction grid and 32-channel multianodes)
Emission Filters (λ/Bandwidth (example fluorophore))
- Detector 1 (PMT)
- 452/45 (DAPI)
- 482/35 (CFP)
- Detector 2 (GaAsP PMT)
- 525/50 (GFP)
- 540/30 (GFP narrow)
- Detector 3 (GaAsP PMT)
- 595/46 (mCherry)
- Detector 4 (PMT)
- 700/75 (Cy5)
Objectives
- 10x Plan Apo λ DIC N1 Air NA 0.45 WD 4,000 µm
- 20x Plan Apo VC DIC N2 Air NA 0.75 WD 1,000 µm
- 40x Apo λS DIC N2 Water NA 1.25 WD 200-160 µm
- 60x Plan Apo DIC N2 IR Water NA 1.27 WD 180-160 µm
- 40x Plan Flour DIC N2 H Oil NA 1.3 WD 240 µm
- 60x Plan Apo λ DIC N2 Oil NA 1.4 WD 130 µm
Time-resolved imaging module
- Pulsed excitation lasers (FRET donor): 440 (CFP-like) & 485 nm (GFP-like)
- Time-correlated single photon counting: MultiHarp 150
- Data acquisition and analysis software SymPhoTime 64
- Dual channel detection filter-setups for multi-color FLIM (λ/Bandwidth)
- CFP/YFP
- 483/40 bandpass
- 488 lpxr beamsplitter
- 550/49 bandpass
- GFP/mCherry
- 520/40 bandpass
- 560 lpxr beamsplitter
- 600/50 bandpass
- GFP/Cy5
- 520/35 bandpass
- 635 lpxr beamsplitter
- 690/70 bandpass
Further information:
https://bua.openiris.io/Landing/Resource?id=5800Possible user: No restrictions
Conditions of use:
The User and PI hereby declare to have understood and accepted the AMBIO User Guidelines and Rules
including the Safety Rules, as available online at: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2025-01-30.pdf. It is further
understood that these Guidelines and Rules may be updated or changed in agreement with the AMBIO
steering committee. Registered Users and PIs will be notified of essential changes (service fees).
Furthermore, the new User and PI have understood that a fee will be charged for the usage of AMBIO services
as outlined in the AMBIO User Guidelines and Rules, and that they are willing and able to pay the fees.
Note
New users – access upon training request here.
Email: ambio@charite.de
Provider:
Advanced Medical BIOimaging Core Facility
Provider Location:
Campus Charité Mitte (CCM)
CharitéCrossOver (CCO)
Rm 01-306
Virchowweg 6
10117 Berlin
Germany
Location:
Charité Campus Mitte
CCO Rm. 01-307
Virchowweg 6
10117 Berlin
Germany