AMBIO Zeiss Upright LSM 900 Airyscan 2
AMBIO-satellite at Charité Campus Mitte (CCM) institute of cell biology and neurobiology (CNB) located in the Charité CrossOver (CCO) building.
Charité Campus Mitte
Virchowweg 6
10117 Berlin (Mitte)
CCO Rm. 05-311
Maintenance, training and assistance is done by Dipl.-Ing. Jutta Schüler (AG Prof. Dr. D. Schmitz). Please contact us regarding experimental setup, labelling and sample preparation/mounting (jutta.schueler@charite.de).
Specifications
MAIN FEATURE: Upright confocal laser scanning microscope featuring the Airyscan 2 super-resolution detector for gentle multiplex high-res imaging of fixed samples.
Imaging Modes
- Confocal laser scanning (DAPI, GFP, mCherry, Cy5, transmitted light)
- Super-resolution laser scanning (DAPI, GFP, mCherry, Cy5)
- Widefield epifluorescence (DAPI, GFP, mCherry, Cy5)
- Bright-field (transmitted light)
- DIC for 25x & 63x objectives
Special
- Super-resolution & higher scan-speed with Airyscan 2 detector (32x-array-detector for pixel re-assignment), 4 color-filtered, optimal with the 40x multi immersion objective (water, silicone, glycerol)
- Multiplex SR-2Y (increased speed with parallel scanning of 2 lines)
- Motorized XYZ stages
- Z-Piezo for super-fast focusing & stacking (500 µm range)
- ZEN experiment-designer for optimal channel/ filter settings & acquisition speed
- Airyscan Joint Deconvolution
Excitation Lasers
- 405 nm
- 488 nm
- 561 nm
- 640 nm
Detectors (range)
- 2x GaAsP detectors: Confocal laser scanning with max. 2 channels simultaneously, within 400 – 700 nm flexible range and width
- 1x Airyscan 2 super-resolution detector (400 – 700 nm flexible range and width, e.g. DAPI, GFP, Cy3, Cy5)
- 1x transmitted light detector (T-PMT)
- Monochromatic fluorescence camera Axiocam 503 mono (1963×1460 pixels, 4.54 µm pixel size)
Additional Emission Filters (Bandwidth (example fluorophore))
- Short λ
- SP 470 (DAPI)
- SP 545 (GFP)
- SP 620 (Cy3)
- No emission filter
- Long λ
- LBF 640 (long range)
- LP 575 (Cy3)
- LP 655 (Cy5)
- SP 620 (GFP, CY3)
- No emission filter
Objectives
- 2.5x Plan-Apochromat (air), NA 0.085, WD 8,800 µm
- 10x Plan-Apochromat (air), NA 0.45, WD 2,100 µm
- 20x Plan-Apochromat (air), NA 0.8, WD 550 µm
- 25x LD LCI DIC Plan-Apochromat (multi immersion: water, silicone, glycerol, oil), NA 0.8, WD 570 µm
- 40x LD LCI Plan-Apochromat (multi immersion: water, silicone, glycerol), NA 1.2, WD 410 µm, à Airyscan super-resolution mode optimized for use of this objective
- 63x Plan-Apochromat DIC (oil), NA 1.4, WD 190 µm
Product page: https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-900-with-airyscan-2.html
Further information:
https://bua.openiris.io/Landing/Resource?id=23008Possible user: No restrictions
Conditions of use:
The User and PI hereby declare to have understood and accepted the AMBIO User Guidelines and Rules
including the Safety Rules, as available online at: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2025-01-30.pdf. It is further
understood that these Guidelines and Rules may be updated or changed in agreement with the AMBIO
steering committee. Registered Users and PIs will be notified of essential changes (service fees).
Furthermore, the new User and PI have understood that a fee will be charged for the usage of AMBIO services
as outlined in the AMBIO User Guidelines and Rules, and that they are willing and able to pay the fees.
Note
Access upon request of analysis project here.
Email: ambio@charite.de
Provider:
Advanced Medical BIOimaging Core Facility
Provider Location:
Campus Charité Mitte (CCM)
CharitéCrossOver (CCO)
Rm 01-306
Virchowweg 6
10117 Berlin
Germany
Location:
Charité Campus Mitte
CCO Rm. 05-311
Virchowweg 6
10117 Berlin
Germany