Imaging modes
- Scanning confocal (including XZ direction scans)
- Wide field (DAPI, FITC, YFP, RHOD, TXR)
- Video-imaging up to 60 fps
- TauSense, TauSeparation, TauGating: functional imaging based on arrival times
- FRAP
- Live-cell incubator (temperature, CO2, humidity)
- Spectral scans
- Lightning mode (near super-resolution)
Lasers and max. intensities out of 60x oil objective
- 405 nm
- White light laser: 440-700 nm (pulsed)
Emission Filters
| Filter | λ/Bandwidth |
| DAPI | 460/50 |
| FITC | 527/30 |
| YFP | 535/30 |
| RHOD | 585/40 |
| TXR | 630/75 |
Scanner
- AOBS scanner
Detectors
- 2x Power Hybrid Detektor HyD S
- 2x Power Hybrid Detektor HyD X
- Transmission light detector (DIC, phase)
- Spectral detection
Objectives
| Mag. | Type | Immer. | NA | WD [μm] | Info |
| 10x | HC PL FLUOTAR | Air | 0.3 | 11,000 | |
| 20x | HC PL FLUOTAR | Air | 0.55 | ||
| 20x | HC PL APO | Water (multi imm) | 0.75 | 670 | |
| 40x | HC PL APO | Oil | 1.30 | 240 | |
| 63x | HC PL APO | Water | 1.20 | 300 | motCorr |
| 2.5x | HC PL FLUOTAR | Air | 0.07 | 9,200 |
Special
- Super Z-galvo stage
- High precision LMT scanning stage
- Automatic water immersion for long-term imaging
- Resonant scanner for fast imaging
Further information:
https://bua.openiris.io/Landing/Resource?id=16807
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Possible user: No restrictions
Note
To get access to the microscope please click on the request access icon.
Email: Dr. Anita Balázs (anita.balazs@charite.de)
Provider:
Shared Equipment at Charité
Provider Location:
Berlin
Location:
Charité Campus Virchow Klinikum
AG Mall
Forschungshaus Rm. 1.0516
Forum 4
Augustenburger Platz 1
13353 Berlin
Germany